Cell culture - thawing and maintaining cell lines using sterile technique. Treatment of cell lines with soluble chemicals and harvesting for qPCR, western blot, and ELISA/related assays to quantify intracellular molecular concentrations. Generation of stable CRISPR/Cas9 and over-expression cell lines. Genotyping of floxed genes and presence of Cre recombinase from mouse tail/ear tissue. Handling mice, administration of drugs subcutaneously and intra-peritoneal, and euthanasia. Perform readouts for animal studies: PCR, Western blot, tissue staining. Assist with flow-cytometry and RNA seq preparation. Ordering supplies/reagents, maintaining buffers, pouring gels, hood maintenance, autoclave. Will assist in the conception, logistical design, and troubleshooting of both in vitro and in vivo experiments. Analyze data from experiments using Excel, Prism, Image J, FlowJo, etc. Use of relevant biostatistics. Other duties as assigned.